Nanostructured Based Lab-on-Chips for Optical and Electrical Detection of Biomolecules
Leslie Dan Faculty of Pharmacy
University of Toronto
9 AM, Tuesday, February 28th, 2017
3102 Ghausi Hall (Civil Engineering Conference Room)
Abstract: Nanostructured materials and nanofabricated lab-on-chip devices are playing crucial roles in genetic diagnosis and sensitive pathogen detection. Identifying genetic disorders or disease states requires dynamic manipulation and trapping of a small number of biomolecules of the target nucleic acids. Current sensing and detection technologies are limited either by sensor resolution or by low throughput in trapping molecules at the detection sites. Inspired by the state of the art nanofabrication and nano-synthesis methods, my research is focused on designing and fabricating lab-on-chip devices and nanostructured sensors to overcome these limitations.
In order to enhance the capturing sites for detection of biomolecules in low-concentration analytes (e.g. Dopamine), we developed metallic/metal oxide nanostructures with an extended surface area for electro-chemical and biochemical reactions. We have adopted a similar approach to develop a nano/microstructured platform to efficiently immobilize DNA and proteins, as part of assays to directly detect a wide range of biomolecules and bacteria.
To further improve trapping efficiency for long nucleic acid molecules, we developed a sample-delivery system based on reversible, tunable nanofluidic confinement of biomolecules based on the di-electrophoretic force using nano/micropatterned electrodes. The resulting device can concentrate and manipulate molecules at high throughput; and can be easily interfaced with integrated optical/electrical detectors. The ease of fabrication and uncomplicated instrumentation makes this patent pending technology a unique point of care instrument for optical and electrical detection of biomolecules.
Biography: Sara Mahshid is a postdoctoral fellow at the University of Toronto in the Leslie Dan Faculty of Pharmacy. Her research interest is nanomaterials (nanotubes/nanowires)-based biosensing and nano/microfluidic lab-on-a-chip devices for biomedical application such as single cell/ single DNA molecule analysis and nanoscale bimolecular detection. She has published over twenty articles including publications in Lab-on-a-chip, Analyst and PNAS. Sara was a postdoc at McGill University (2012-2015), jointly appointed by the Department of Physics and the Human Genetics Department. Before starting her postdoc research at McGill, she was a full time lecturer (teaching and research) at the Engineering Department of Monash University Sunway Campus, Malaysia. Sara earned her MSc and PhD degrees in Materials Science and Engineering at Sharif University of Technology, Iran in 2011. She has over ten years of experience in research, teaching, mentoring and supervision.